- Title
- Increased neuronal differentiation of neural progenitor cells derived from phosphovimentin-deficient mice
- Creator
- Chen, Meng; Puschmann, Till B.; Marasek, Pavel; Inagaki, Masaki; Pekna, Marcela; Wilhelmsson, Ulrika; Pekny, Milos
- Relation
- Molecular Neurobiology Vol. 55, Issue 7, p. 5478-5489
- Publisher Link
- http://dx.doi.org/10.1007/s12035-017-0759-0
- Publisher
- Springer
- Resource Type
- journal article
- Date
- 2018
- Description
- Vimentin is an intermediate filament (also known as nanofilament) protein expressed in several cell types of the central nervous system, including astrocytes and neural stem/progenitor cells. Mutation of the vimentin serine sites that are phosphorylated during mitosis (VIMSA/SA) leads to cytokinetic failures in fibroblasts and lens epithelial cells, resulting in chromosomal instability and increased expression of cell senescence markers. In this study, we investigated morphology, proliferative capacity, and motility of VIMSA/SA astrocytes, and their effect on the differentiation of neural stem/progenitor cells. VIMSA/SA astrocytes expressed less vimentin and more GFAP but showed a well-developed intermediate filament network, exhibited normal cell morphology, proliferation, and motility in an in vitro wound closing assay. Interestingly, we found a two- to fourfold increased neuronal differentiation of VIMSA/SA neurosphere cells, both in a standard 2D and in Bioactive3D cell culture systems, and determined that this effect was neurosphere cell autonomous and not dependent on cocultured astrocytes. Using BrdU in vivo labeling to assess neural stem/progenitor cell proliferation and differentiation in the hippocampus of adult mice, one of the two major adult neurogenic regions, we found a modest increase (by 8%) in the fraction of newly born and surviving neurons. Thus, mutation of the serine sites phosphorylated in vimentin during mitosis alters intermediate filament protein expression but has no effect on astrocyte morphology or proliferation, and leads to increased neuronal differentiation of neural progenitor cells.
- Subject
- intermediate filaments; nanofilaments; vimentin; GFAP; astrocytes; neural stem/progenitor cells; Bioactive3D culture system
- Identifier
- http://hdl.handle.net/1959.13/1393636
- Identifier
- uon:33582
- Identifier
- ISSN:0893-7648
- Rights
- © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:/ / creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Language
- eng
- Full Text
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